1268 The association between novel LncRNA expression levels by in situ hybridization method and stages of patients with cutaneous melanoma

High mortality of cutaneous melanoma occurred usually at terminal stage; therefore, identifying the molecular mechanisms that regulate metastasis and developing useful therapeutic strategies are crucial tasks. Long non-coding RNAs (LncRNAs), noncoding transcripts >200 nucleotides in length, showed important roles on regulating cancer cellular processes, including metastasis. Previous our study lncRNA (linc00094) a possible role oncogene. LINC00094-knockdown cells (A2058 A375)showed significantly decreased cell proliferation, colony formation, migration, invasion ability impair cycle progression by cycle-related genes. The objective this is to unravel association linc00094 expression paraffin section pathological stages those patients with melanoma. intensity lnc00094 paraffine were presented performing “In situhybridization (ISH) (RNAscope)”technology. Compare ISH Linc00094 tissues, recurrent adjacent normal epidermis, control (melanocytic nevus) significant differences (P<0.05). was related TNM level gradually increases stage I (mean± SD: 2.1±2.34) III (2.51±2.66) but IV (1.496±2.31) decreases even lower than I. N0(2.28±2.41) N1(2.93±3.11) increased, N1 N3(1.18±2.10) decreases, M0(2.32±2.46) M1(1.496±2.13) also decreases. above finding supported an early marker oncogene from (I, II, III). seems loss its function as stage. levels inspired further investigation.